Free donation - New coronavirus SARS-CoV-2 nucleic acid testing method

(Enhanced fluorescence
real-time reverse-transcription PCR (ERT-PCR))


In response to the new coronavirus pneumonia (CoronaVirus Disease 2019, COVID-19) outbreak, over one hundred nucleic acid-based kits and methods for detection of the novel coronavirus SARS-CoV-2 have been reported. After one and a half month of clinical application, many problems with the kits were exposed as reflected in the appearance of significant numbers of false negatives. This caused the public to question the application of nucleic acid detection methods for screening and diagnosis. In addition, inconsistent standards were used in the development of the tests, the performance indicators provided by different kit suppliers could not be compared, and large differences were found in the detection performance of the different kits. Therefore, detection methods for SARS-CoV-2 urgently need to be optimized to cope with the ongoing epidemic.


Hai Kang Life Technology Corporation Limited offers a nucleic acid detection method for the detection of SARS-CoV-2: enhanced fluorescence real-time reverse- transcription PCR (ERT-PCR). Compared with general RT-qPCR, the method has ~10 times greater sensitivity, which can alleviate the false negative problem found in current clinical nucleic acid tests and improve the accuracy of detection.


As the COVID-19 outbreak has become a Public Health Emergency of International Concern, Hai Kang would like to donate its in-house developed test to non-profit organizations around the world. (If you will be using the method for commercial use, please contact us directly.)


 We believe that appropriate test methods can be selected for different groups of people to achieve the purposes of screening, diagnosis and confirmation. The ERT-PCR method described herein, due to its high sensitivity, can be applied to screening of people in isolation or of the general community (ensuring screening reliability) or post-recovery verification (avoiding false negatives). If needed, we can also provide training to technicians with relevant operational experience so as to achieve maximum diagnostic capacity in emergency situations.

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